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Dosage GuideResearch Reference

TB-500 Dosage

Research-based dosing protocols, timing guidance, and reconstitution reference for TB-500. All information is for educational purposes only.

Quick Answer

TB-500 (Thymosin Beta-4 fragment) is typically researched at 2.0–2.5 mg per injection, administered subcutaneously or intramuscularly 2 times per week during a 4–6 week loading phase, followed by a maintenance dose of 1–2 mg once weekly. These dosing ranges derive from animal studies and community protocols; no human clinical dose-ranging trials have been completed.

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Table of Contents

Standard Dosage Range

Research dosing range: 2.0–2.5 mg per injection (loading); 1.0–2.0 mg per injection (maintenance)

Educational reference only

These dosage ranges are derived from preclinical research and community protocols. No human clinical dose-ranging trials have established therapeutic doses for most research peptides. Always consult a qualified healthcare provider before starting any peptide protocol.

Dosage by Use Case

Acute Muscle / Tendon Injury (Loading Phase)

2.0–2.5 mg2× per week
Duration

4–6 weeks

Loading phase designed to saturate tissue with TB-500. Injections can be subcutaneous at any site — TB-500 has high systemic mobility due to its small molecular size. Pair with BPC-157 for synergistic connective tissue repair.

Maintenance / Performance Optimization

1.0–2.0 mg1× per week
Duration

Ongoing, typically 4–8 weeks cycles

After loading phase, reduce frequency to maintain elevated tissue levels. Lower doses sufficient for maintenance once initial repair processes are initiated.

Cardiac Support (Preclinical)

2.5 mg2× per week
Duration

4 weeks post-event

Animal studies of myocardial infarction used TB-500 to reduce fibrosis and promote repair. Not validated in humans. Included for research reference only.

Hair Regrowth Protocol

1.0–2.0 mg2× per week
Duration

8–12 weeks

Preliminary evidence from animal models showing TB-500 activates hair follicle stem cells. Human hair regrowth evidence is largely anecdotal.

Timing & Frequency

TB-500 has an estimated half-life of 6–8 hours. Twice-weekly injections maintain reasonably stable tissue concentrations during loading phases. Timing relative to workouts or injury events does not appear critical based on available research — systemic distribution allows the peptide to reach injury sites regardless of injection location.

Cycle Guidance

Standard protocol: 4–6 week loading phase (2–2.5 mg twice weekly), followed by 4–8 week maintenance (1–2 mg once weekly). Common research cycles: 10–12 weeks total, then 4–6 weeks off. TB-500 does not affect the hypothalamic-pituitary axis, so hormone-related cycling rationale does not apply. Cycling is recommended due to limited long-term human safety data.

Reconstitution Reference

Quick reference for reconstituting TB-500. For custom vial sizes and concentrations, use the Reconstitution Calculator.

Common Vial SizeTypically 2 mg or 5 mg per vial
BAC Water Volume1 mL bacteriostatic water per 2 mg vial (for 2 mg/mL)
Concentration & DrawAt 2 mg/mL: 2 mg dose = 1 mL (100 units on insulin syringe). At 5 mg/2.5 mL: 2 mg = 0.8 mL (80 units).
StorageRefrigerate at 2–8°C after reconstitution. Lyophilized powder can be stored at room temperature.
StabilityReconstituted: up to 30 days refrigerated. Lyophilized: 12–24 months.
Use the Reconstitution Calculator → for precise injection volumes based on your exact vial size, water volume, and desired dose.

Frequently Asked Questions

What is the typical TB-500 dosage for muscle recovery?
The most common research protocol uses 2.0–2.5 mg twice per week for 4–6 weeks (loading phase) for acute muscle injuries. For ongoing recovery optimization, a maintenance dose of 1–2 mg once weekly is commonly used after the loading phase. These protocols derive from community experience and animal research — no human clinical trials exist.
Should TB-500 be injected near the injury site?
TB-500 has exceptional systemic mobility — it is small enough to travel through tissues and reach injury sites from any injection point. Unlike BPC-157, localized injection near the injury is not considered critical. Most protocols use subcutaneous injection at the abdomen or thigh for convenience. The peptide distributes systemically and accumulates at sites of inflammation.
How do I calculate TB-500 injection volume?
Use the Reconstitution Calculator (/tools/reconstitution-calculator). Example: 5 mg vial + 2.5 mL BAC water = 2 mg/mL concentration. For a 2 mg dose: 2 ÷ 2 = 1 mL = 100 units on an insulin syringe. For a 2.5 mg dose: 2.5 ÷ 2 = 1.25 mL (use a 1.5 mL syringe for this volume).
Can I stack TB-500 with BPC-157?
Yes — this is one of the most popular research stacks. BPC-157 and TB-500 work through complementary mechanisms: BPC-157 targets localized repair via GHR upregulation and VEGF angiogenesis, while TB-500 promotes systemic tissue repair via actin upregulation and cell migration. The combination is commonly used for significant soft tissue injuries. Typical protocol runs both simultaneously during the loading phase.
How long does TB-500 take to show effects?
Anecdotal reports suggest subjective improvements in 1–2 weeks, with more substantial recovery by week 4–6. Animal wound healing studies show measurable histological improvements within 2 weeks. Joint and tendon injuries may take the full 6–8 week cycle. Hair regrowth effects, if any, typically manifest over 8–12 weeks.
What is the difference between TB-500 dosage and Thymosin Beta-4 dosage?
TB-500 is a synthetic fragment of the full Thymosin Beta-4 protein, containing the active actin-binding domain. Dosing conventions for TB-500 are essentially the same as those developed for Thymosin Beta-4 in research. The available commercial form is almost exclusively TB-500 (the fragment), not the full Tβ4 protein, due to cost and manufacturing considerations.

References

  1. 1
    Thymosin beta4 sulfoxide is an anti-inflammatory agent generated by monocytes in the presence of glucocorticoids(2000)PubMed ↗
  2. 2
    Thymosin beta4 is a potent regulator of tumor cell motility and migration(2006)PubMed ↗
  3. 3
    The role of thymosin beta 4 in blastema formation and activation during salamander limb regeneration(2003)PubMed ↗
  4. 4
    Thymosin beta4: a multi-functional regenerative peptide. Basic properties and clinical applications(2014)PubMed ↗
  5. 5
    Thymosin beta4 promotes the repair of spinal cord injury through a functional recovery study in rats(2016)PubMed ↗

Last updated: 2026-02-26