Vilon Research

Ta HM, Sankaran B, Roush ED, Ferreon JC, Ferreon ACM, Kim C et al.

Fyn is a Src-family tyrosine kinase implicated in synaptic dysfunction and neuroinflammation across multiple neurodegenerative disorders, including Alzheimer's disease (AD) and Parkinson's disease (PD). Saracatinib (AZD0530) is a potent Src-family inhibitor that has been explored as a repurposed ...

Liu Y, Nadig I, Mehta AS, Chuo SW, Ho YH, Tyler J et al.

The formation of stable protein complexes enables much of biotechnology, but even high-affinity complexes can dissociate, limiting potential applications in biomaterials, bioimaging, nanomedicine, and other protein-based technologies. Here, we describe lock coupling, a simple and selective one-st...

Gong R, Chen F, Liu B, Wang Y

Wheat protein hydrolysates (WPHs) can chelate calcium; however, their chelation mechanism remains unclear. This study aimed to isolate and identify novel calcium-chelating peptides using liquid chromatography-tandem mass spectrometry (LC-MS/MS), and then to synthesize them using a solid-phase pro...

Yao H, Luo C, Xian Y, Liu B, Yang T, Tian R et al.

Primary idiopathic male infertility remains a significant challenge due to its complex etiology and the lack of effective diagnostic tools. This study characterized seminal microbiota and metabolome alterations in this condition and their association with sperm quality, aiming to identify potenti...

Dobriţescu A, Samide A, Cioateră N, Mic OC, Ionescu C, Dăbuleanu I et al.

Experimental and theoretical studies were applied to investigate the adsorption properties of testagen (KEDG) peptide on copper surfaces in sodium chloride solution and, implicitly, its inhibition efficiency (IE) on metal corrosion. The tetrapeptide synthesized from the amino acids lysine (Lys), ...

Patil G, Frasko P, Lier B, Gabler T, Furtmüller PG, Oostenbrink C et al.

Active site architectures of enzymes are defined by many interactions between substrate and amino acid residues and are optimized for specific and efficient substrate turnover. In the case of coproheme decarboxylase (ChdC) the active site architecture is well described by structural and thermodyn...

Zhu W, Huang Y, Wang H, Zhang Z, Gao X, Lin H et al.

Limited research on epitopes restricts further investigation and application of the sarcoplasmic calcium binding proteins (SCP). In this study, linear epitopes in Portunus trituberculatus SCP (Por t 4) were identified and compared with different SCPs. Variations in Por 4 epitopes under different ...

Walla B, Dietrich AM, Brames E, Bischoff D, Fritzsche S, Castiglione K et al.

Industrial biotechnology offers a potential ecological solution for PET recycling under relatively mild reaction conditions via enzymatic degradation, particularly using the leaf branch compost cutinase (LCC) quadruple mutant ICCG. To improve the efficient downstream processing of this biocatalys...

Ibrahim RB, Vispo B, Fasipe T, Devaraj S

Over 1400 variants of hemoglobin (Hb) have been identified and characterized with phenotypes ranging from clinically silent to severe clinical manifestations in carriers. Different analytical methods have been established to detect Hb variants. Here, we report the first pediatric case of hemoglob...

Kraskovskaya N, Linkova N, Sakhenberg E, Krieger D, Polyakova V, Medvedev D et al.

Neurons become more vulnerable to stress factors with age, which leads to increased oxidative DNA damage, decreased activity of mitochondria and lysosomes, increased levels of p16, decreased LaminB1 proteins, and the depletion of the dendritic tree. These changes are exacerbated in vulnerable neu...

Hasegawa S, Inose R, Igarashi M, Tsurumaki M, Saito M, Yanagisawa T et al.

Cold-shock proteins (Csps), of around 70 amino acids, share a protein fold for the cold-shock domain (CSD) that contains RNA-binding motifs, RNP1 and RNP2, and constitute one family of bacterial RNA-binding proteins. Despite similar amino acid composition, Csps have been shown to individually pos...

Bharti M, Sharma M, Choksket S, Khurana H, Siwach S, Modeel S et al.

A rod-shaped, motile, Gram-stain-positive bacterial strain RKN2T, was isolated from gut of silver carp (Hypophthalmichthys molitrix) residing in Gobindsagar reservoir, Himachal Pradesh, India. Having the greatest sequence similarity to Sporosarcina koreensis F73T (98.51%), Sporosarcina luteola Y1...

Chertkova RV, Oleynikov IP, Pakhomov AA, Sudakov RV, Semenova MA, Arutyunyan AM et al.

Cytochrome c (CytC), a one-electron carrier, transfers electrons from complex bc1 to cytochrome c oxidase (CcO) in the electron-transport chain. Electrostatic interaction with the partners, complex bc1 and CcO, is ensured by a lysine cluster near the heme forming the Universal Binding Site (UBS)....

Ilina AR, Popovich IG, Ryzhak GA, Khavinson VK

Alzheimer's disease (AD) is the most common neurodegenerative disorder, characterized by progressive cognitive decline. This review discusses current therapeutic strategies for the treatment of Alzheimer's disease, their limitations, and potential prospects. The feasibility of comprehensive appro...

Anderson JW, Vaisar D, Jones DN, Pegram LM, Vigers GP, Chen H et al.

Activation of the extracellular signal-regulated kinase-2 (ERK2) by phosphorylation has been shown to involve changes in protein dynamics, as determined by hydrogen-deuterium exchange mass spectrometry (HDX-MS) and NMR relaxation dispersion measurements. These can be described by a global exchang...

Shang YW, Chen X, Wu YS, Zhou ZX, Liu Y, Liu YR et al.

This study aims to explore the mechanism of Liujunzi Decoction in the treatment of 4-nitroquinoline-N-oxide(4NQO)-induced esophageal cancer in mice. One hundred mice of 35-45 days were randomized into blank, model, and low-, medium-, and high-concentration(18.2, 36.4, and 54.6 g·kg~(-1), res...

Fan H, Shang N, Davidge ST, Wu J

This study aims to investigate the antihypertensive effect of four chicken muscle-derived angiotensin (Ang)-converting enzymes (ACE)-regulating peptides: Val-Arg-Pro (VRP, ACE inhibition), Leu-Lys-Tyr and Val-Arg-Tyr (LKY and VRY, ACE inhibition and ACE2 upregulation), and Val-Val-His-Pro-Lys-Glu...

Cai Y, He L, Cao S, Zeng P, Xu L, Luo Y et al.

The study aimed to compare the effects of crystalline L-lysine and L-glutamate (CAA), Lys-Glu dipeptide (KE) on the growth and muscle development of grass carp (Ctenopharyngodon idellus), and related molecular mechanisms. Five experimental diets (CR, 0.5% CAA, 1.5% CAA, 0.5% KE, 1.5% KE) containi...

Xu Q, Chen H, Zhu J, Li X, Yang J, Deng X et al.

In this study, the PEG-Glu-Lys-Glu copolymer drug delivery system (GO/PEG-Glu-Lys-Glu) is prepared using glutamate-lysine-glutamate (Glu-Lys-Glu) modified polyethylene glycol (PEG) and connected graphene oxide nanosheets (GO). The multiple carboxyl groups of Glu-Lys-Glu and π-π intera...

Khavinson VK, Linkova NS, Ashapkin VV, Shilovsky GA, Borushko NV, Petukhov MG et al.

It was shown that KE peptide (Lys-Glu, vilon) has immunomodulatory, oncostatic and geroprotective effects. The aim of this work is to evaluate the effect of the KE peptide on gene expression and protein synthesis of SIRT1, PARP1, PARP2 during aging of human mesenchymal stem cells (MSC). The KE pe...