Crystagen Research

Li Z, Li Y, Zhao Y, Wang G, Liu R, Li Y et al.

Promoting the synchronization of glucose and amino acid release in the digestive tract of pigs could effectively improve dietary nitrogen utilization. The rational allocation of dietary starch sources and the exploration of appropriate dietary glucose release kinetics may promote the dynamic bala...

Khavinson V, Linkova N, Kozhevnikova E, Dyatlova A, Petukhov M

Ultrashort peptides (USPs), consisting of 2-7 amino-acid residues, are a group of signaling molecules that regulate gene expression and protein synthesis under normal conditions in various diseases and ageing. USPs serve as a basis for the development of drugs with a targeted mechanism of action....

Khavinson VK, Kopylov AT, Vaskovsky BV, Ryzhak GA, Lin'kova NS

The polypeptide complex of the epiphysis and the peptide AEDG, constructed on the basis of its amino acid analysis, exert similar biological effects. Both bioregulators normalize melatonin synthesis in the pineal gland, functioning of the brain, eye retina, cardiovascular, endocrine, and immune s...

Сhervyakova NA, Linkova NS, Chalisova NI, Koncevaya EA, Trofimova SV, Khavinson VK et al.

Vilon, Timogen, Crystagen and R-1 short peptides possess various immunoprotective effects in spleen during its ageing. Both R-1 and Vilon peptides activate T-helpers. The effect of Vilon is provided by decreased level of apoptosis, as well as the effect of R-1 peptide is provided by increased pro...

Linkova NS, Khavinson VKh, Chalisova NI, Katanugina AS, Koncevaya EA

We studied cell composition of the pineal lymphoid tissue and the effect of peptides on its differentiation and proliferation capacity. It was shown that the lymphoid component of the pineal gland in organotypic culture is primarily presented by low-differentiated CD5(+)-lymphocytes, while mature...

Khavinson VKh, Nikolsky IS, Nikolskaya VV, Zubov DA, Galickaya SN, Taranuha LI et al.

Tripeptides T-36 and, particularly, T-38 in concentrations of 0.1, 1, and 10 ng/ml inhibited proliferation of primary trypsinized embryonic mesenchymal stem cells, rat transplantable KF-1 fibroblasts, and human erythromyelosis K-562 cells. Inhibition of proliferation in embryonic and immortalized...

Ubiyvovk VM, Ananin VM, Malyshev AY, Kang HA, Sibirny AA

Tripeptide glutathione (gamma-glutamyl-L-cysteinyl-glycine) is the most abundant non-protein thiol that protects cells from metabolic and oxidative stresses and is widely used as medicine, food additives and in cosmetic industry. The methylotrophic yeast Hansenula polymorpha is regarded as a rich...

Kim EY, Ro YT, Kim YM

Ribulose 1,5-bisphosphate carboxylases/oxygenases (RuBisCOs) of two carboxydobacteria, Acinetobacter sp. strain JC1 and Hydrogenophaga pseudoflava, grown on carbon monoxide were purified and partially characterized. RuBisCO of Acinetobacter sp. JC1 was purified 5-fold in eight steps to homogeneit...

Wang Y, Darnay BG, Rodwell VW

Kinetic analysis of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase has implicated a glutamate or aspartate residue in (i) formation of mevaldate thiohemiacetal by proton transfer to the carbonyl oxygen of mevaldate and (ii) enhanced ionization of CoASH by the resulting enzyme carboxyla...

Raffioni S, Luporini P, Chait BT, Disper SS, Bradshaw RA

The complete amino acid sequence of the mating pheromone Er-1 purified from Euplotes raikovi homozygous for mat-1 was determined by automated Edman degradation of the whole protein and peptides generated by cyanogen bromide, trypsin, Staphylococcus aureus V8 protease, and chymotrypsin. The propos...

Thampy KG, Huang WY, Wakil SJ

A rapid method for the purification of pyruvate carboxylase from rat liver has been developed. The method involves extraction of the enzyme from frozen liver powder followed by polyethylene glycol fractionation and avidin-affinity chromatography. The purified enzyme has a specific activity of 9-1...

Wang CS

A procedure for the purification of carboxyl ester lipase from human pancreas has been developed. The determined N-terminal 10 amino acid residues of the purified enzyme, NH2-Ala-Lys-Leu-Gly-Ala-Val-Tyr-Thr-Glu-Gly, was identical to the terminal of human milk bile salt-activated lipase. The human...

Rittenhouse J, Harrsch PB, Kim JN, Marcus F

Fructose-1,6-bisphosphatase from the yeast Saccharomyces cerevisiae has properties similar to other gluconeogenic fructose-1,6-bisphosphatases, but an unusual characteristic of the yeast enzyme is that it can be phosphorylated in vitro by cAMP-dependent protein kinase. Phosphorylation also occurs...

Baram GI, Grachev MA, Nazimov IV, Pletnev AG, Pressman EK

Protein E of the tick-borne encephalitis virus (TBEV) (strain Sofin) was treated with purified trypsin in 1% Triton X-100, and the peptides thus obtained were separated by micro-column reversed-phase chromatography. Four of the purified peptides were sequenced, their structures being in accordanc...

Wang CS, Johnson K

A modified procedure for a large-scale purification of human milk bile salt-activated lipase (BAL) has been devised. An initial step used cholate-Sepharose affinity chromatography for the partial purification of the enzyme followed by the removal of cholate with a Bio-Rex 5 anion-exchange resin. ...

Voskuyl-Holtkamp I, Schattenkerk C

A description is given of the synthesis by fragment condensation of the peptide Gly-Glu-Ser-Arg-Glu-Ser-Ser-Ala-Asp-Lys-Phe-Lys-Arg-Gln-His-Met-Asp-Thr-Glu-Gly-Pro-Ser-Lys corresponding to the 1--23 amino acid sequence of rat pancreatic ribonuclease. This rat peptide combined with bovine S-protei...

Hargrave PA

A glycopeptide (T1) composed of 16 amino acids has been isolated from a tryptic digest of bovine rhodopsin. Its sequence is Met-Asn(CHO)-Gly-Thr-Glu-Gly-Pro-Asn-Phe-Tyr-Val-Pro-Phe-Ser-Asn(CHO)-Lys. Both rhodopsin and peptide T1 are blocked at their amino terminals. When a method specific for iso...

Hargrave PA, Fong SL

The amino terminus of bovine rhodopsin is blocked and has the sequence x-Met-Asn(CHO)-Gly-Thr-Glu-Gly-Pro-Asn-Phe-Tyr-Val-Pro-Phe-Ser-Asn(CHO)-Lys-Thr-Gly-Val-Val-Arg, where CHO represents sites of carbohydrate attachment. The carboxyl-terminal sequence of rhodopsin is Val-Ser-Lys-Thr-Glu-Thr-Ser...

Han YN, Kato H, Iwanaga S, Suzuki T

On incubation of bovine plasma high-molecular-weight (HMW) kininogen with purified plasma kallikrein [EC 3.4.21.8], a large glycopeptide fragment and the vasoactive peptide, bradykinin, were initially liberated; the former, named fragment 1-2, was subsequently cleaved into fragment 1 (glycopeptid...