Alarelin Research

Yuan F, Zhang P, Liu M, Li Y, Xu B, Li X et al.

Alarelin acetate, a synthetic gonadotropin-releasing hormone (GnRH) analogue, is widely used to manage endometriosis and hormone-sensitive malignancies. Although its safety profile is generally favorable, we report the first documented case of severe hepatotoxicity associated with alarelin acetat...

Alijani A, Niasari-Naslaji A, Sayyah N, Baninajar M, Ganjkhanlou M

It is desirable in estrus synchronization in sheep to avoid intravaginal devices and to shorten the program from 14 to 6 days. Moreover, replacement of eCG with safe, cheap, and efficient gonadotropin is in worldwide demand.This study investigates the possibility of replacing eCG with human recom...

Podhorec P, Knowles J, Vysloužil J, Boryshpolets S, Kubová K, Rodina M et al.

Carp pituitary treatment versus poly (lactiac-co-glycolic acid) microparticles with slow release of Alarelin at 35 µg kg-1 or 200 µg kg-1 body weight to induce spermiation was compared in sterlet Acipenser ruthenus. All hormone treatments initially increased testosterone and 11-ketotest...

Zhang K, Gao G, Zhao X, Li Q, Zhong H, Xie Y et al.

1. The study objectives were to determine the direct effects of gonadotropin-releasing hormone (GnRH) on the proliferation of ovarian granulosa cells (GCs) and the development of follicles in geese (Anser cygnoides) by colorimetry and ethynyl-2'-deoxyuridine (EdU) cell proliferation assays, in wh...

Feng K, Cui X, Song Y, Tao B, Chen J, Wang J et al.

Gonadotropin-releasing hormone (Gnrh) plays important roles in reproduction by stimulating luteinizing hormone release, and subsequently ovulation and sperm release, ultimately controlling reproduction in many species. Here we report on a new role for this decapeptide. Surprisingly, Gnrh3-null ze...

Zhang Z, Li R, Liu S, Yin L, Xu T, Fawcett JP et al.

Alarelin, a gonadotropin-releasing hormone analogue, is widely used in China for the treatment of endometriosis and uterine leiomyoma. In order to investigate its pharmacokinetic behavior and support the preclinical application of new formulations, we have developed a novel and highly selective b...

Nojavan S, Bidarmanesh T, Mohammadi A, Yaripour S

In the present study, for the first time electromembrane extraction followed by high performance liquid chromatography coupled with ultraviolet detection was optimized and validated for quantification of four gonadotropin-releasing hormone agonist anticancer peptides (alarelin, leuprolide, busere...

Zhang S, Han J, Leng G, Di X, Sha C, Zhang X et al.

A liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) method was developed, using testosterone-d3 as a surrogate analyte, for the simultaneous quantification of goserelin and testosterone in rat plasma. According to this method, the pharmacokinetic and pharmacody...

Wei S, Chen S, Gong Z, Ouyang X, An L, Xie K et al.

We investigated the effects of gonadotropin releasing hormone (GnRH) agonist on expressions of GnRH receptor (GnRHR), follicle-stimulating hormone receptor (FSHR) and luteinizing hormone receptor (LHR) proteins in the ovaries and follicular development in the ewes. Forty-two pre-pubertal ewes wer...

Duan JL, Jiang YH, Liu Y, Zeng QF, Huang YD

To compare the pituitary down-regulatory effects of the two gonadotropin-releasing hormone agonists Alarelin and Triptorelin in the long protocol of ovulation induction in in vitro fertilization and embryo transfer (IVF-ET).We included in this study 122 patients aged 24-39 years treated by IVF-ET...

Zhan Y, Chen X, Zhao X, Zhong D

Leuprolide is a synthetic nonapeptide that has two basic amino acids, arginine and histidine, in its structure. By selection of an appropriate analytical column and optimizing the mobile phase composition, an improved analytical method has been developed and validated to determine leuprolide conc...

Chen L, Yin SD, Sun XD

To study the effects of Gonadotropin-releasing hormone-I (GnRH-I) analog alarenin on inositol 1, 4, 5-triphosphate (IP(3)) in gastric parietal cells of rats in vitro.The gastric parietal cells of rats were isolated and incubated in vitro and alarenlin at various concentration were added to the me...

Pan YM, Shi YF, Chen HZ

To evaluate the influence of superovulation by GnRHa protocol and pregnant mare's serum gonadotropin (PMSG) alone on the expression of estrogen receptor (ER), progesterone receptor (PR) and leukemia inhibitory factor (LIF) mRNA on endometrium.Forty-five female ICR mice were randomly allocated int...

Kong Y, Zhang JW

To evaluate the therapeutic effect of Tamoxifen in conjunction with a cell apoptosis contributor--Rhizoma curcumae oil on the rat endometriosis models.The rat endometriosis models were established and were randomly assigned into the control group, Tamoxifen group, Alarelin (GnRHa) group, and the ...

Chen L, Sun XD, Zhao J, Yang AG, Huang WQ

Our objective was to study the distribution of gonadotropin-releasing hormone (GnRH) and its receptor, cloning and sequencing of GnRH and its receptor gene in cultured gastric parietal cells of rats. The distribution of GnRH and its receptor mRNA were investigated through immunocytochemical ABC m...

Chen L, He HX, Sun XD, Zhao J, Liu LH, Huang WQ et al.

To investigate the expression of gonadotropin-releasing hormone (GnRH) receptor and the effects of GnRH analog (alarelin) on proliferation of cultured gastric smooth muscle cells (GSMC) of rats.Immunohistochemical ABC methods and in situ hybridization methods were used to dectect protein and mRNA...