Humanin Dosage Guide: Research Protocols, Timing & Reconstitution

Standard Dosage Range

Research dosing range: 5–20 mcg/kg daily (subcutaneous, estimated)

Educational reference only

These dosage ranges are derived from preclinical research and community protocols. No human clinical dose-ranging trials have established therapeutic doses for most research peptides. Always consult a qualified healthcare provider before starting any peptide protocol.

Dosage by Use Case

Neuroprotection (Alzheimer's Prevention)

10-20 mcg/kg1× daily

Duration

4-12 weeks

Based on rat models of amyloid-beta toxicity. Subcutaneous injection. Co-administration with other nootropics is sometimes explored.

Insulin Sensitivity & Glucose Regulation

5-10 mcg/kg1× daily

Duration

4-8 weeks

Targeting IGFBP-3 modulation. Subcutaneous injection. Monitoring blood glucose is advisable.

Cardioprotection (Ischemia-Reperfusion)

10-15 mcg/kg1× daily

Duration

4-6 weeks

Preclinical evidence in animal models of myocardial infarction. Subcutaneous injection. Still very early stage research.

General Cytoprotection & Anti-Aging

5 mcg/kg1× daily

Duration

4-8 weeks

Lower dose for systemic benefits. Subcutaneous injection. Consider cycling to mitigate potential long-term effects.

Timing & Frequency

Subcutaneous injections are typically administered in the morning. Shorter half-life may warrant split doses, but this is not widely practiced. S14G-Humanin (HNG) has a longer half-life.

Cycle Guidance

Given the limited human data, cycling is often recommended. A common approach is 8 weeks on, 4 weeks off. Monitor for any adverse effects during on-cycles.

Reconstitution Reference

Quick reference for reconstituting Humanin. For custom vial sizes and concentrations, use the Reconstitution Calculator.

Common Vial Size	Typically 1 mg per vial
BAC Water Volume	1 mL bacteriostatic water for 1 mg/mL concentration
Concentration & Draw	At 1 mg/mL: 10 mcg/kg requires careful calculation based on body weight.
Storage	Refrigerate at 2–8°C after reconstitution. Keep lyophilized powder at room temperature or refrigerated.
Stability	Reconstituted solution stable up to 14 days refrigerated. Lyophilized powder stable 12-24 months per manufacturer specifications.

Use the Reconstitution Calculator → for precise injection volumes based on your exact vial size, water volume, and desired dose.

Frequently Asked Questions

What is the optimal dosage of Humanin for neuroprotection?

Based on preclinical studies, a range of 10-20 mcg/kg daily, administered subcutaneously, is often cited for neuroprotective effects. However, no human clinical trials have validated this dosage.

Can Humanin be taken orally?

Oral bioavailability of Humanin is thought to be very low due to its peptide structure. Subcutaneous injection is the preferred route of administration. Analogs like S14G-Humanin may offer improved stability and potentially better oral bioavailability, but more research is needed.

What are the potential side effects of Humanin?

Given the limited human data, the side effect profile of Humanin is not well-established. Potential side effects may include injection site reactions, mild flu-like symptoms, and altered glucose metabolism. Monitoring blood glucose is recommended.

How should Humanin be stored?

Lyophilized Humanin should be stored at room temperature or refrigerated. After reconstitution with bacteriostatic water, the solution should be refrigerated at 2-8°C and is typically stable for up to 14 days.