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Dosage GuideResearch Reference

AOD-9604 Dosage

Research-based dosing protocols, timing guidance, and reconstitution reference for AOD-9604. All information is for educational purposes only.

Quick Answer

AOD-9604 is typically administered via subcutaneous injection at dosages of 250-500 mcg once daily, often in the morning on an empty stomach. For fat loss, a 12-20 week cycle is common. Some protocols use a higher initial dose (500 mcg) for 1-2 weeks. Intra-articular injection is experimental.

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Table of Contents

Standard Dosage Range

Research dosing range: 250-500 mcg per dose (subcutaneous); 200-400 mcg (intra-articular)

Educational reference only

These dosage ranges are derived from preclinical research and community protocols. No human clinical dose-ranging trials have established therapeutic doses for most research peptides. Always consult a qualified healthcare provider before starting any peptide protocol.

Dosage by Use Case

Fat Loss / Weight Management

250-300 mcg1× daily
Duration

12-20 weeks

Subcutaneous injection into abdominal fat, ideally in the morning on an empty stomach. Some users cycle AOD-9604 with other fat-loss agents.

Loading Protocol (Fat Loss)

500 mcg1× daily
Duration

1-2 weeks, then reduce to 250 mcg

Higher initial dose for rapid saturation. Limited clinical evidence supports this approach. Monitor for increased side effects.

Osteoarthritis / Cartilage Repair (Investigational)

200-400 mcg1-2× weekly
Duration

Varies

Intra-articular injection by a medical professional. Not FDA-approved. Limited evidence. May be combined with hyaluronic acid injections.

Timing & Frequency

For fat loss, AOD-9604 is usually administered in the morning, approximately 30 minutes before breakfast. This is thought to optimize lipolytic effects during the fasted state. For intra-articular use, timing is less critical.

Cycle Guidance

For fat loss, AOD-9604 cycles typically last 12-20 weeks. There is no established cycling protocol. Some practitioners recommend cycling off for a period equal to the on-cycle duration to maintain sensitivity. For cartilage repair, treatment duration depends on clinical response.

Reconstitution Reference

Quick reference for reconstituting AOD-9604. For custom vial sizes and concentrations, use the Reconstitution Calculator.

Common Vial SizeTypically 2 mg per vial
BAC Water Volume1 mL bacteriostatic water for 2 mg/mL concentration
Concentration & DrawAt 2 mg/mL: 250 mcg = 0.125 mL (12.5 units on a 100-unit insulin syringe)
StorageRefrigerate at 2-8°C after reconstitution. Keep lyophilized powder at room temperature or refrigerated.
StabilityReconstituted solution stable up to 14 days refrigerated. Lyophilized powder stable 12-24 months per manufacturer specifications.
Use the Reconstitution Calculator → for precise injection volumes based on your exact vial size, water volume, and desired dose.

Frequently Asked Questions

What is the best time to inject AOD-9604 for fat loss?
Most protocols recommend subcutaneous injection in the morning on an empty stomach, approximately 30 minutes before eating. This is believed to maximize its lipolytic effects in a fasted state, though formal studies confirming this are lacking.
Where should I inject AOD-9604?
For fat loss, subcutaneous injection into abdominal fat is common. Rotating injection sites is recommended to prevent localized irritation. For intra-articular use, injection is directly into the joint space by a qualified medical professional only.
How long does AOD-9604 take to work?
Some users report noticeable fat loss within 4-6 weeks of consistent use. Clinical trials showed statistically significant effects after 12 weeks. Results depend on diet, exercise, and individual response. It is not a "magic bullet" and requires lifestyle modifications for optimal effects.
Can I combine AOD-9604 with other peptides for fat loss?
Yes, AOD-9604 is sometimes combined with other peptides. However, there is limited clinical data on these combinations. Peptide stacking should only be done under medical supervision due to the increased risk of unpredictable effects.
What is the correct dosage for AOD-9604?
The standard dosage for subcutaneous injection is 250-500 mcg once daily. Some protocols start with 500 mcg for 1-2 weeks and then reduce to 250 mcg. Intra-articular dosages (200-400 mcg) are experimental. It is essential to start with a low dose and monitor for side effects.

References

  1. 1
    A synthetic fragment of human growth hormone (AOD9604) that reduces body fat in obese Zucker rats(2001)PubMed ↗
  2. 2
    AOD9604, a novel lipolytic peptide fragment of human growth hormone: safety, tolerability, and pharmacokinetics in healthy subjects(2003)PubMed ↗
  3. 3
    Metabolic effects of a synthetic lipolytic domain (AOD9604) of human growth hormone(2000)PubMed ↗

Last updated: 2026-02-19